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Team:Alberta/References/Publications/Folding and unfolding single RNA molecules under tension
From 2009.igem.org
Michael T Woodside, Cuauhtemoc Garcia-Garcia and Steven M Block
Current Opinion in Chemical Biology Volume 12, Issue 6, December 2008, Pages 640-646
Abstract: Single-molecule force spectroscopy constitutes a powerful
method for probing RNA folding: It allows the kinetic, energetic,
and structural properties of intermediate and transition states
to be determined quantitatively, yielding new insights into
folding pathways and energy landscapes. Recent advances in
experimental and theoretical methods, including fluctuation
theorems, kinetic theories, novel force clamps, and ultrastable
instruments, have opened new avenues for study. These tools
have been used to probe folding in simple model systems, for
example, RNA and DNA hairpins. Knowledge gained from such
systems is helping to build our understanding of more complex
RNA structures composed of multiple elements, as well as how
nucleic acids interact with proteins involved in key cellular
activities, such as transcription and translation.
Link: [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6VRX-4TDBD62-3&_user=1067472&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000051251&_version=1&_urlVersion=0&_userid=1067472&md5=86de56ff39356e353cb1a05791719f23 ScienceDirect]
